Interfacing Biology and Physical Sciences by Microfluidic and Nanoelectronic Circuits: Our research interests are to develop microfluidics-based technology platforms and to utilize these technology platforms to address important issues in the fields of molecular imaging, cancer biology and chemistry. In contrast to conventional bench-top setups, microfluidics has been used to manipulate chemical and biological processes on nanoliter (nL) to microliter (mL) scales with the profound advantages of enhanced heat transfer performance, faster diffusion time and reaction kinetics, as well as improved control over experimental conditions. By incorporating integrated microvalves into microfluidic batch systems, the integrated microfluidic platforms enable the execution and automation of multiple, parallel, and/or sequential chemical or biological processes on a single chip under digital control. For the past two years, we have been working with several research groups who have expertise covering molecular imaging, cancer biology, immunology and pathology to develop a variety of functioning microfluidic platforms for applications in three major areas of interest:
Development of Molecular Imaging Probes
Production of Nanostructured Materials
In a boarder playing field, our goal remains the creation of further integrated microfluidic technology platforms for the broader space in chemistry and biology beyond a single functioning platform on a chip. We will compile many different microfluidic platforms to produce a number of game systems: “Let’s Play _____”, where the blank is “systems biology of disease”, “biomarkers”, “pharmacology”, “biochemical reaction kinetics”, “chemistry” and “cancer diagnosis” etc. where execution is rapid and learning curves are steep.
- Chen K.J., Tang L., Garcia M.A., Wang H., Lu H., Lin W.Y., Hou S., Yin Q., Shen C.K., Cheng J., Tseng H.R., “The therapeutic efficacy of camptothecin-encapsulated supramolecular nanoparticles,” Biomaterials. 2012 Feb;33(4):1162-69. Epub 2011 Nov 8.
- Vu N.T., Yu Z.T., Comin-Anduix B., Sondergaard J.N., Silverman R.W., Chang C.Y., Ribas A., Tseng H.R., Chatziioannou A.F., “A beta-camera integrated with a microfluidic chip for radioassays based on real-time imaging of glycolysis in small cell populations,” J Nucl Med. 2011 May;52(5):815-21.
- Wang S., Owens G.E., Tseng H.R., “Nano “fly paper” technology for the capture of circulating tumor cells,” Methods Mol Biol. 2011;726:141-50.
- Liu Y., Wang H., Kamei K., Yan M., Chen K.J., Yuan Q., Shi L., Lu Y., Tseng H.R., “Delivery of intact transcription factor by using self-assembled supramolecular nanoparticles,” Angew Chem Int Ed Engl. 2011 Mar 21;50(13):3058-62. Epub 2011 Mar 2.
- Chen K.J., Wolahan S.M., Wang H., Hsu C.H., Chang H.W. Durazo A., Hwang L.P. Garcia M.A., Jiang Z.K., Wu L., Lin Y.Y., Tseng H.R., “A small MRI contrast agent library of gadolinium(III)-encapsulated supramolecular nanoparticles for improved relaxivity and sensitivity,” Biomaterials. 2011 Mar;32(8):2160-65. Epub 2010 Dec 16.
- Kamei K., Sun J., Tseng H.R., Damoiseaux R., “Microfluidic image cytometry,” Methods Mol Biol. 2011;706:191-206.
- Liu K., Chen Y.C., Tseng H.R., Shen C.K., van Dam R.M., “Microfluidic device for robust generation of two-component liquid-in-air slugs with individually controlled composition,” Microfluid Nanofluidics. 2010 Oct;9(4-5):933-43. Epub 2010 Apr 22.
- Fang C., Wang Y., Vu N.T., Lin W.Y., Hsieh Y.T., Rubbi L., Phelps M.E., Muschen M., Kim Y.M., Chatizzoannou A.F., Tseng H.F., Graeber T.G., “Integrated microfluidic and imaging platform for a kinase activity radioassay to analyze minute patient cancer samples,” Cancer Res. 2010 Nov 1;70(21):8299-308. Epub 2010 Sep 13.
- Sun J., Masterman-Smith M.D., Graham N.A., Jiao J., Mottahedeh J., laks D.R. Ohashi M., DeJesus J., Kamei K., Lee K.B., Wang H., Yu Z.T., Lu Y.T., Hou S., Li K., Liu M., Zhang N., Wang S., Angenieux B., Panosyan E., Samuels E.R., Park J., Williams D., Konkankit V., Nathanson D., van Dam R.M., Phelps M.E., Wu H., Liau L.M., Mischel P.S. lazareff J.A., Kornblum H.I., Yong W.H., Graeber T.G., Tseng H.R., “A microfluidic platform for systems pathology: multiparameter single-cell signaling measurements of clinical brain tumor specimens,” Cancer Res. 2010 Aug 1;70(15):6128-38. Epub 2010 Jul 14.
- Wang S., Chen K.J., Wu T.H., Wang H., Lin W.Y., Ohashi M., Chiou P.Y. Tseng H.R., “Photothermal effects of supramolecularly assembled gold nanoparticles for the targeted treatment of cancer cells,” Angew Chem Int Ed Engl. 2010 May 17;49(22):3777-81.