Harold Monbouquette Phd


Department of Chemical and Biomolecular Engineering

310-825-8946 | 310-206-4107 (fax)


  • AB, Harvard College, 1979
  • BS, University of Massachusetts, Amherst, 1982
  • MS, University of California, Davis, 1984
  • PhD, North Carolina State University, 1987


  • Department of Energy Environmental Restoration and Waste Management Young Faculty Award, 1990
  • TRW Excellence in Teaching Award, 1990-91

Research Interests

Molecular engineering of innovative
systems that mimic the biological (i.e., biomimetic systems) in efforts to
solve technological problems.

Future advances in many areas of
interest to chemical engineers including catalysis, advanced materials, and
separations will require engineering at the molecular scale. Since the direct
manipulation of individual molecules presents obvious technological
difficulties, much research has focused on self-assembling systems. The living
cell is the ultimate self-assembled system with unrivaled capabilities in
separations, sensing and synthesis; and lipid bilayer membranes are critical
self-assembled structures for cell functioning as they serve, for example, to
define cell boundaries, to compartmentalize reactions in controlled
environments, and to provide scaffolding for the molecular machines involved in
signal and energy transduction. My research projects on vesicular biomimetic
membranes for separations, metal ion detection and nanoparticle synthesis; and
on lipid-modified electrodes for biosensor development and for electroenzymatic
chiral synthesis were conceived with the attributes of cell membranes in mind.
Another common theme in my research, therefore, is the reliance on
self-assembly to produce new technology that is critically dependent on
molecular-scale structure. 


Recent Papers

  1. Chiu M.L., Ytterberg A.J., Ogorzalek Loo R.R., Loo J.A., Monbouquette H.H., “Characterization of morphine-glucose-6-phosphate dehydrogenase conjugates by mass spectrometry,” Bioconjug Chem. 2011 Aug 17;22(8):1595-604. Epub 2011 Jul 7.
  2. Chiu M.L., Tseng T.T., Monbouquette H.G., “A convenient homogeneous enzyme immunoassay for estradiol detection,” Biotechnol Appl Biochem. 2011 Jan-Feb;58(1):75-82.
  3. Radhakrishnan C., Lo M.K., Knobler C.M., Garcia-Garibay M.A., Monbouquette H.G., “Capping-Ligand Effect on the Stability of CdSe Quantum Dot Langmuir Monolayers,” Langmuir. 2011 Feb 17. (Epub ahead of print)
  4. Chiu M.L., Lai D., Monbouquette H.G., “An influenza hemagglutinin A peptide assay based on the enzyme-multiplied immunoassay technique,” J Immunoassay Immunochem. 2011 Jan;32(1):1-17.
  5. Galatsis K., Wang K.L., Ozkan M., Ozkan C.S., Huang Y. Chang J.P., Monbouquette H.G., Chen Y., Nealey P., Botros Y., “Patterning and templating for nanoelectronics,” Adv mater. 2010 Feb 9;22(6):769-78.
  6. Goldsmith L.E., Pupols M., Kickhoefer V.A., Rome L.H., Monbouquette H.G., “Utilization of a protein “shuttle” to load vault nanocapsules with gold probes and proteins,” ACS Nano. 2009 Oct 27;3(10):3175-83.
  7. Lai D., Lluncor B., Schroder I., Gunsalus R.P., Liao J.C., Monbouquette H.G., 
    Reconstruction of the archaeal isoprenoid ether lipid biosynthesis pathway in Escherichia coli through digeranylgeranylglyceryl phosphate,” Metab Eng. 2009 may;11(3):184-91. Epub 2009 Feb 12.
  8. Lim S., Springstead J.R., Yu M., Bartkowski W., Schroder I., Monbouquette H.G., “Characterization of a key trifunctional enzyme for aromatic amino acid biosynthesis in Archaeoglobus fulgidus,” Extremophiles. 2009 Jan;13(1):191-8. Epub 2008 Dec 11.
  9. Yu M., Ng B.C., Rome L.H., Tolbert S.H., Monbouquette H.G., “Reversible pH lability of cross-linked vault nanocapsules,” Nano Lett. 2008 Oct;8(10):3510-5. Epub 2008 Sep 20. 
  10. Ng B.C., Yu M., Gopal A., Rome L.H., Monbouquette H.G., Tolbert S.H,. “Encapsulation of semiconducting polymers in vault protein cages,” Nano Lett. 2008 Oct;8(10):3503-9. Epub 2008 Sep 20.